Adsorption behaviors of recombinant E-cadherin-IgG Fc fusion protein on polystyrene surface

作者：Sakai S; Kim J; Hexig B; Okahata Y; Cho C S; Akaike T^*

来源：Colloids and Surfaces B: Biointerfaces , 2012, 94: 192-198.

DOI：10.1016/j.colsurfb.2012.01.031

• Adsorption
• E-cad-Fc
• Quartz-crystal microbalance
• Deglycosylation
• Homophilic adhesion

Adsorption behaviors of recombinant E-cadherin-IgG Fc (E-cad-Fc) fusion protein and mutated E-cad-Fcs on the polystyrene (PS) surface were investigated using a 27 MHz quartz-crystal microbalance (QCM) and ELISA. The amount of adsorbed E-cad-Fc on PS surface was increased with an increase of E-cad-Fc concentration as a Langmuir-type in a monolayer. Adsorbed E-cad-Fc on PS surface was stable even after washing if calcium ions are absent in the washing solution due to the calcium ion dependence in the adsorption. E-cadherin homophilic adhesion among E-cadherins during adsorption of E-cad-Fc was involved. Deglycosylation of the E-cad in the E-cad-Fc did not affect adsorption of E-cad-Fc on the PS surface although deglycosylation of the E-cad in the E-cad-Fc enhanced cell adhesion compared with E-cad-Fc.

• 出版日期2012-6-1