Detection of HBV RNA in peripheral blood mononuclear cells in patients with and without HBsAg by reverse transcription polymerase chain reaction

作者：Mei, SD; Yatsuhashi, H^*; Parquet, MD; Hamada, R; Fujino, T; Matsumoto, T; Inoue, O; Koga, M; Yano, M

来源：Hepatology Research, 2000, 18(1): 19-28.

DOI：10.1016/S1386-6346(99)00081-9

• hepatitis B virus (HBV)
• peripheral blood mononuclear cells (PBMCs)
• HBV RNA
• reverse transcription polymerase chain reaction (PT-PCR)
• polymerase chain reaction (PCR)

We examined RNA of hepatitis B virus (HBV) in peripheral blood mononuclear cells (PBMCs) by the reverse transcription polymerase chain reaction (RT PCR) in 61 patients associated with HBV infection, in order to analyze the relationship between the transcriptional activity of HBV in PBMCs and the clinical characteristics. The presence of HBV RNA in PBMCs was detected in 19/51(37.1%) patients with HBsAg positive and in 1/10 (10.0%) patient with HBsAg negative patients. Six healthy controls were all negative. The frequency of HBV RNA positivity was detected in patients with high ALT level (P < 0.05), serum HBeAg positivity (P < 0.01) and serum HBV DNA level greater than or equal to 0.7 Meq/ml (P < 0.05). Moreover, HBV RNA in PBMCs was detected in one patient followed up for 2 years after HBsAg disappearance in serum, who had not HBV DNA but anti-HBc IgG, in serum. These results suggested that the transcription of HBV in PBMCs, was frequently detected in the patients with higher replication of the virus, but HBV RNA in PBMCs might be detected in a few patients who had no evidence of HBV replication serologically.

• 出版日期2000-7
• 单位上海市闵行区中心医院