A recombinant DNA and vaccinia virus prime-boost regimen induces potent long-term T-cell responses to HCV in BALB/c mice

作者：Deng Yao; Zhang Ke; Tan Wenjie^*; Wang Yue; Chen Hong; Wu Xiaobing; Ruan Li

来源：Vaccine, 2009, 27(15): 2085-2088.

DOI：10.1016/j.vaccine.2009.02.003

• Hepatitis C virus
• T-cell response
• Prime-boost regimen

To explore the best prime-boost regimen and evaluate the T-cellular response memory against HCV, we constructed two DNA vaccine candidates (pVRC-CE1E2 and pAAV-CE1E2) and two recombinant viruses (rTTV-E1E2 and rAAV-E1E2) and then assessed the immune response to different prime-boost patterns in BALB/c mice. The rTTV-E1E2 boosted the immune response to HCV DNA vaccine prime significantly, and the inverted terminal repeat sequence harboring DNA construct PAAV-CE1E2 was the best prime agent in this study. Our study provides new information for both the prime-boost regimen and long-term T-cell response for HCV vaccine development.

• 出版日期2009-3-26
• 单位中国疾病预防控制中心; 中国疾病预防控制中心病毒病预防控制所