Emergence of Escherichia coli Sequence Type 131 Isolates Producing KPC-2 Carbapenemase in China

作者：Cai, Jia Chang; Zhang, Rong; Hu, Yan Yan; Zhou, Hong Wei; Chen, Gong-Xiang^*

来源：Antimicrobial Agents and Chemotherapy, 2014, 58(2): 1146-1152.

DOI：10.1128/AAC.00912-13

• HYDROLYZING BETA-LACTAMASE
• KLEBSIELLA-PNEUMONIAE
• SERRATIA-MARCESCENS
• PORIN EXPRESSION
• CLINICAL ISOLATE
• RESISTANCE
• STRAIN
• CLONE
• ENTEROBACTERIACEAE
• CTX-M-15

Twenty-two KPC-2-producing Escherichia coli isolates were obtained from three hospitals in Hangzhou, China, from 2007 to 2011. One isolate, with OmpC porin deficiency, exhibited high-level carbapenem resistance. Pulsed-field gel electrophoresis showed that few isolates were indistinguishable or closely related. Multilocus sequence typing indicated that sequence type 131 (ST131) was the predominant type (9 isolates, 40.9%), followed by ST648 (5 isolates), ST405 (2 isolates), ST38 (2 isolates), and 4 single STs, ST69, ST2003, ST2179, and ST744. Phylogenetic analysis indicated that 9 group B2 isolates belonged to ST131, and 5 of 11 group D isolates belonged to ST648. Only one group B1 isolate and one group A isolate were identified. A representative plasmid (pE1) was partially sequenced, and a 7,788-bp DNA fragment encoding Tn3 transposase, Tn3 resolvase, ISKpn8 transposase, KPC-2, and ISKpn6-like transposase was obtained. The bla(KPC-2)-surrounding sequence was amplified by a series of primers. The PCR results showed that 13 isolates were consistent with the genetic environment in pE1. It is the first report of rapid emergence of KPC-2-producing E. coli ST131 in China. The bla(KPC-2) gene of most isolates was located on a similar genetic structure.

• 出版日期2014-2
• 单位浙江大学