Analysis of the peri-implant microbiota in 90 dental implants and its relationship to crevicular fluid volume

作者：Ata Ali Javier; Juan Flichy Fernandez Antonio; Alegre Domingo Teresa; Eugenia Candel Marti Maria; Penarrocha Oltra David; Francisco Balaguer Martinez Jose; Penarrocha Maria^*

来源：Medicina Oral Patologia Oral y Cirugia Bucal, 2011, 16(7): C944-C947.

DOI：10.4317/medoral.17231

• Tannerela forsythia
• Porphyromonas gingivales
• Treponema denticola
• Aggregatibacter actinomycetemcomitans
• peri-implant microbiota
• crevicular fluid volume
• smoking

Objective: To evaluate the presence within the peri-implant sulcus of Tannerela forsythia (Tf), Porphyromonas gingivales (Pg), Treponema denticola (Td) and Aggregatibacter actinomycetemcomitans (Aa), and relate these bacteria to the peri-implant crevicular fluid volume (PICFV).
Material and Method: A prospective and cross-sectional clinical case series study was made. For the measurement of crevicular fluid, use was made of the Periotron (R) 8000 (Proflow Incorporated. New York, USA), measuring the volume in Periotron units (PU). For the detection of periodontopathogenic bacteria we used the IAI-PadoTest 4.5 (IAI Inc., IAI Institute, Zuchwil, Switzerland) - a system for the detection of Tf, Pg, Td and Aa based on the use of RNA arrays.
Results: We included 34 patients (19 females and 15 males) with a mean age of 56.4 years. Of these subjects, 30.8% were smokers and 69.2% non-smokers. Out of a total series of 213 implants, we analyzed the crevicular fluid and microbiota in 90 implants. A total of 16.5% of the implants presented mucositis, while 83.5% were in healthy peri-implant conditions. The microbiological study revealed the presence of Tf in 17.1% of the implants, Pg in 9.3%, Td in 13.6%, in Aa in none of the implants. The mean Periotron reading was 93.4 PU (range 12-198 PU). A statistically significant (p<0.05) relationship was observed between PICFV and the total percentage bacteria (Tf, Pg and Td) - with a strong association between the Td levels and smoking (p<0.01). In the implants with mucositis, the concentration of Pg and Td was greater.
Conclusions: In the implants studied, the subgingival peri-implant microbiota was characterized by low levels of Pg, Tf, Td, and none of the patients proved positive for Aa. These bacteria showed a positive correlation to crevicular fluid volume, and a statistically significant relationship was observed between Td and smoking.

• 出版日期2011-11