Application of FTIR Spectrometry to Monitoring Chemosensitivity of Breast Cancer Cell Line Using 5-FU

作者：Hu Hai-jian; Zu Chao; Guo Lin; Hu Qing-nan; Wang Jian-sheng^*; Xu Yi-zhuang; Wu Jin-guang

来源：Spectroscopy and Spectral Analysis, 2013, 33(11): 2935-2939.

DOI：10.3964/j.issn.1000-0593(2013)11-2935-05

• Breast cancer cell
• 5-FU
• Infrared spectroscopy
• Monitor

The purpose of the present study was to compare the changes of infrared spectrum of breast cancer cell line MCF-7 treated by 5-fluorouracil with various concentrations and treating time. The results indicate that the relative intensity ratios of I-2 920/I-1 460 increased in the FTIR spectra of cell line MCF-7 while I-1 400/I-1 460, I-1 080/I-1 550 and I-1 240/I-1 550 decreased significantly within forty-eight hours, however, no regular change was observed during 48 similar to 72 hours. The relative intensity ratio of I-1 640/I-1 550 decreases along with the increase in the concentration of 5-fluorouracil. The changes of infrared spectra are consistent with the biochemical changes in breast cancer cell, and it can be the reference designators when assessing curative effect of breast cancer cell by FTIR spectrometry.

• 出版日期2013-11
• 单位北京大学; 辽宁中医药大学; 西安交通大学