Muscarinic Acetylcholine Receptor Subtype Expression in Type Vestibular Hair Cells of Guinea Pigs

作者：Yao, Qi; Cheng, Huamao; Guo, Changkai; Zhou, Tao; Huang, Xiang; Kong, Weijia^*

来源：Journal of Huazhong University of Science and Technology-Medical Sciences, 2011, 31(5): 682-686.

DOI：10.1007/s11596-011-0582-1

• muscarinic acetylcholine receptor
• type. vestibular hair cells
• single-cell RT-PCR

Recent studies have demonstrated that five subtypes (M1-M5) of muscarinic acetylcholine receptor (mAChR) are expressed in the vestibular periphery. However, the exact cellular location of the mAChRs is not clear. In this study, we investigated whether there is the expression of M1-M5 muscarinic receptor mRNA in isolated type. vestibular hair cells of guinea pig by using single-cell RT-PCR. In vestibular end-organ, cDNA of the expected size was obtained by RT-PCR. Moreover, mRNA was identified by RT-PCR from individually isolated type. vestibular hair cells (single-cell RT-PCR). Sequence analysis confirmed that the products were M1-M5 mAChR. These results demonstrated that M1-M5 mAChR was expressed in the type. vestibular hair cells of the guinea pig, which lends further support for the role of M1-M5 mAChR as a mediator of efferent cholinergic signalling pathway in vestibular hair cells.

• 出版日期2011-10
• 单位华中科技大学