MicroRNA-182-5p Ameliorates Liver Ischemia-Reperfusion Injury by Suppressing Toll-Like Receptor 4

作者：Jiang, W.^*; Liu, G.; Tang, W.

来源：Transplantation Proceedings, 2016, 48(8): 2809-2814.

DOI：10.1016/j.transproceed.2016.06.043

• GRAFT INJURY
• CANCER CELLS
• INNER-EAR
• MIR-182
• EXPRESSION
• INHIBITION
• ACTIVATION
• IMMUNITY
• LIGANDS
• MIR-96

Background. Toll-like receptor 4 (TLR4) has received a lot of attention in studies on ischemia-reperfusion (I/R) injury. TLR4 is one of the target genes for microRNA-182-5p (miR-182-5p)-mediated regulation. In this study, we evaluated the role(s) of miR-182-5p transcriptional regulation in a hepatic I/R injury model in vivo and a lipopolysaccharide (LPS)-induced inflammatory reaction model in vitro. @@@ Methods. The effects of miR-182-5p expression on the transcription and translation of TLR4 and several proinflammatory cytokines in a LPS-induced macrophage model were studied in vitro. After miR-182-5p was exogenously administered, the transcriptional/ translational profile of TLR4 and the proinfiammatory cytokines were evaluated in an in vivo I/R injury model. This data were combined with alanine transaminase expression levels and histologic features in an effort to understand the role(s) of miR-182-5p in a liver I/R model. @@@ Results. TLR4 mRNA and protein levels in LPS-induced macrophages were significantly decreased in the presence of miR-182-5p (P < .01). In vivo TLR4 mRNA and protein levels as well as the induction of several proinflammatory cytokines, including tumor necrosis factor a and interleukin-6, were decreased in the presence of miR-182-5p. Hematoxylin and eosin staining showed that edema and necrosis in I/R modes were alleviated in the miR-182-5p-expressing group compared with control. @@@ Conclusions. miR-182-5p attenuates hepatic I/R by directly suppressing TLR4.

• 出版日期2016-10
• 单位南京医科大学