Differential developmental programming by early protein restriction of rat skeletal muscle according to its fibre-type composition

作者：da Silva Aragao R; Guzman Quevedo O; Perez Garcia G; Toscano A E; Gois Leandro C; Manhaes de Castro R; Bolanos Jimenez F^*

来源：Acta Physiologica, 2014, 210(1): 70-83.

DOI：10.1111/apha.12073

• developmental programming
• metabolism
• skeletal muscle

AimsDifferences in fibre-type composition of skeletal muscle have been associated with obesity and insulin resistance. As a poor nutrient environment early in life is a predisposing factor for the development of obesity and related metabolic diseases at adulthood, this study aimed at determining the long-term consequences of maternal undernutrition on the structural and metabolic properties of two skeletal muscles characterized by their different fibre-type composition and metabolic properties. %26lt;br%26gt;MethodsThe fibre-type composition and enzymatic activities of hexokinase (HK), beta-hydroxyacyl-CoA dehydrogenase (-HAD) and citrate synthase (CS) were measured in soleus and extensor digitorum longus (EDL) muscles from adult rats born to dams fed a control (17% protein) or a low-protein [8% protein (PR)] diet throughout pregnancy and lactation. In addition, the expression levels of several genes regulating glycolysis, fatty acid oxidation and mitochondrial biogenesis were determined by real-time PCR. %26lt;br%26gt;ResultsProtein rats exhibited enhanced density of type II fibres along with decreased rate of fatty acid oxidation and glycolysis in soleus but not EDL. Malnourished rats exhibited also a different gene expression profile in soleus and EDL. Altogether, these alterations correspond to a state of energy deficiency and are present in animals which do not show yet any sign of obesity or glucose intolerance. %26lt;br%26gt;ConclusionWe conclude that maternal protein restriction alters in the long term the structural and enzymatic properties of offspring skeletal muscle in a fibre-type-dependent manner. These alterations might have a causative role in the development of obesity and related metabolic disorders later in life.

• 出版日期2014-1