Molecular Identification of Cellulose Degrading Trichoderma viride

作者:Sri Puttagunta Udaya*; Kranthi Gampala; Rajasekhar Pinnamaneni
来源:Journal of Pure and Applied Microbiology, 2010, 4(1): 95-102.

摘要

The objective of the present study was to identify the cellulose degrading Trichoderma viride. Dilution plating was used to quantify the propagule numbers of Trichoderma, denaturing gradient gel electrophoresis (DGGE) and DNA sequence analysis were used to identify Trichoderma species. Trichoderma viride was identified based on the microscopic observations by Lactophenol cotton blue and scanning electron microscopy. It was ultimately confirmed based on DGGE and DNA sequence analysis and finally concluded by BLAST analysis by constructing a phylogenetic tree.

  • 出版日期2010-4