Absence of functional TLR4 impairs response of macrophages after Candida albicans infection

作者：Gasparoto Thais Helena; Tessarolli Vanessa; Garlet Thiago Pompermaier; Torres Sergio Aparecido; Garlet Gustavo Pompermaier; da Silva Joao Santana; Campanelli Ana Paula^*

来源：Medical Mycology, 2010, 48(8): 1009-1017.

DOI：10.3109/13693786.2010.481292

• TLR4
• innate response
• Candida albicans

Candida albicans is recognized by phagocytic cells through a set of recognition receptors patterns. Recently, we showed the importance of TLR2 in the regulation of neutrophil survival after C. albicans infection. In the present work, we analyzed the involvement of TLR4 in the recognition of C. albicans by neutrophils and macrophages. Our results show that the absence of functional TLR4 resulted in lower chemotaxis of neutrophils to the site of infection, lower levels of TNF-alpha, CXCL1 and nitric oxide, and dissemination and persistence of the pathogen in lymph nodes and spleen. In vitro, the phagocytic activity, nitric oxide production and myeloperoxidase activity, CXCL1, IL-1 beta production by neutrophils from TLR4-defective mice were not changed. In contrast, macrophages from TLR4-defective mice demonstrated lower phagocytosis and lower levels of CXCL1, IL-1 beta and TNF-alpha. Together, these data demonstrate that TLR4 signals are important for the recognition of C. albicans by macrophages and their absence allows persistence of the infection.</.

• 出版日期2010-12