Involvement of soil bacteria in ABO blood mistyping

作者：Takada Naoki^*; Mori Chikahiro; Takai Rie; Takayama Tomohiro; Watanabe Yoshihisa; Nakamura Kohei; Takamizawa Kazuhiro

来源：Legal Medicine, 2015, 17(2): 128-133.

DOI：10.1016/j.legalmed.2014.10.007

• ABO blood mistyping
• ABO antigen

The current study investigated whether ABO blood mistyping of human biological samples is induced by soil bacteria. A total of 380 bacterial strains were isolated from 50 discrete soil samples using human blood agar, and glycosidase activity evaluated for all strains using 4-nitropheny glycosides (4-nitrophenyl n-acetyl-alpha-D-galactosaminide, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside) as substrates. Thirteen strains possessed alpha-galactosidase activity, and 16S rRNA sequence analysis revealed a close relatedness to the genus Bacillus. An indirect competitive enzyme-linked immunosorbent assay confirmed seven strains exhibited type B antigen degradation activity. These results demonstrated that 1.8% of the bacteria isolated from soil, were Bacillus sp., possessed galactosidase activity, and had the potential to cause ABO blood mistyping.

• 出版日期2015-3