Application of betaine improves solution uptake and in vitro shoot multiplication in tea

作者：Saini Uksha; Kaur Devinder; Chanda Sanjoy; Bhattacharya Amita^*; Ahuja Paramvir Singh

来源：Plant Growth Regulation, 2012, 67(1): 65-72.

DOI：10.1007/s10725-012-9664-8

• Betaine
• Micropropagation
• Tea
• Vascular differentiation
• UPASI cultivars

The application of betaine, a quaternary ammonium compound influenced the micropropagation in two commercially important UPASI (U-9 and U-10) cultivars of tea. Growth and multiplication of shoots of both the cultivars was enhanced significantly in the presence of 125-1,000 mg l(-1) betaine with best response at 1,000 mg l(-1) betaine. The shoots turned brown and died within 15 days when 1,500 mg l(-1) betaine was applied. The study showed faster water/nutrient uptake in the presence of betaine. Higher assimilation of carbon and nitrogen in the presence of betaine was also indicated in biochemical analyses. Thus, a decrease in carbohydrates coupled with an increase in nitrate reductase activity was recorded. Moreover, faster differentiation of vascular elements and shoot thickness was observed in the shoots of U-9 and U-10 growing on medium containing 1,000 mg l(-1) betaine. Nutrient uptake, assimilation and growth were significantly higher in U-10 as compared to U-9 shoots.

• 出版日期2012-5