Anti-biofilm and antimicrobial activity of Mentha pulegium L essential oil against multidrug-resistant Acinetobacter baumannii

作者：Tutar Ugur^*; Celik Cem; Karaman Isa; Atas Mehmet; Hepokur Ceylan

来源：Tropical Journal of Pharmaceutical Research, 2016, 15(5): 1039-U211.

DOI：10.4314/tjpr.v15i5.20

• Mentha pulegium
• Essential oil
• Anti-biofilm
• Antimicrobial activity
• Multi-drug resistance
• A. baumannii

Purpose: To investigate the antimicrobial and anti-biofilm activities of essential oil from Mentha pulegieum L. (EOMP) on multi-drug resistant (MDR) isolates of A. baumannii, as well as its phytochemical composition, antioxidant properties and cytotoxic activity.
Methods: The phytochemical composition of EOMP was analyzed by gas chromatography, while its antimicrobial activities were determined by disc diffusion and broth micro-dilution methods. Minimal biofilm inhibition concentration (MBIC) and minimal biofilm eradication concentration (MBEC) tests were used for assessment of its anti-biofilm properties. Viability in the biofilm was studied using 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay, while colorimetric assay was used to assess its cytotoxicity on L929 cells
Results: D-isomenthone, pulegone, isopulegone, menthol and piperitenone were the major components of the plant extract. EOMP produced > 22 mm inhibition zone for the isolates, with minimum inhibitory concentration (MIC) and MBIC of 0.6 -2.5 and 0.6 -1.25 mu L/mL, respectively, while MBEC was >= 10 mu L/msL. EOMP damaged biofilm structures formed by A. baumannii strains at MIC by 26 - 91 %.
Conclusion: These results suggest that EOMP contains agents that may be useful in the development of new drugs against A. baumannii infections.

• 出版日期2016-5