Global view of transcriptome in the brains of aged NR2B transgenic mice

作者：Li, Chunxia; Su, Men; Wang, Huimin^*; Hu, Yinghe

来源：Neural Regeneration Research, 2013, 8(29): 2734-2743.

DOI：10.3969/j.issn.1673-5374.2013.29.005

• neural regeneration
• memory
• NR2B transgenic mice
• aging
• gene expression
• P53
• insulin-like growth factor
• grants-supported paper
• neuroregeneration

NR2B subunits are involved in regulating aging, in particular, age-related learning and memory deficits. We examined 19-month-old NR2B transgenic mice and their littermate controls. First, we detected expression of the NR2B subunit gene, Grin2b, in the neocortex of transgenic Mice using real-time PCR. Next, we used microarrays to examine differences in neocortical gene expression. Pathway and signal-net analyses identified multiple pathways altered in the transgenic mice, including the P53, Jak-STAT, Wnt, and Notch pathways, as well as regulation of the actin cytoskeleton and neuroactive ligand-receptor interactions. Further signal-net analysis highlighted the P53 and insulin-like growth factor pathways as key regulatory pathways. Our results provide new insight into understanding the molecular mechanisms of NR2B regulated age-related memory storage, normal organismal aging and age-related disease.

• 出版日期2013-10
• 单位华东师范大学