Dietary administration of sodium alginate and kappa-carrageenan enhances the innate immune response of brown-marbled grouper Epinephelus fuscoguttatus and its resistance against Vibrio alginolyticus

作者：Cheng Ann Chang; Chen Yu Yuan; Chen Jiann Chu^*

来源：Veterinary Immunology and Immunopathology, 2008, 121(3-4): 206-215.

DOI：10.1016/j.vetimm.2007.09.011

• Epinephelus fuscoguttatus
• Vibrio alginolyticus
• sodium alginate
• kappa-carrageenan
• respiratory burst
• phagocytic activity
• alternative complement activity
• lysozyme activity

Brown-marbled grouper Epinephelus fuscoguttatus which had been fed diets containing sodium alginate and kappa (K)I carrageenan at 5, 10 and 20 g kg(-1), respectively after 0, 2, 4, 6, 8 and 14 weeks were examined for survival, growth, innate cellular and Immoral responses as compared to the fish that fed control non-supplemented diet. Survival was 100% for the fish that fed all diets after 14 weeks and no significant difference in growth was observed among seven diets. The fish that fed a diet containing sodium alginate at 10 g kg(-1), and fed a diet containing K-carrageenan at 5 g kg(-1) over 2-8 weeks showed significantly increased leucocyte count, respiratory burst, phagocytic activity and phagocytic index, The fish that fed a diet containing sodium alginate at 5 g kg(-1), and fed a diet containing K-carrageenan at 5 g kg(-1) over 2-8 weeks showed significantly increased alternative complement activity (ACH50) and lysozyme activity. In another experiment, E. fuscoguttatus, which had been fed control diet, and all diets containing sodium alginate, and K-carrageenan after 14 weeks were challenged with Vibrio alginolyticus at 5.0 x 10(9) colony-forming units (cfu) fish(-1) and then placed in seawater of 34 parts per thousand. The survival of fish that fed a diet containing sodium alginate at 10 g kg(-1) and K-carrageenan at 5 g kg(-1) was significantly higher than that of fish which fed the control diet over 96-168 h. It was concluded that E. fuscoguttatus which fed a diet containing sodium alginate at 10 g kg(-1) or less, or fed a diet containing kappa-carrageenan at 5 g kg(-1) enhance the innate immunity, and increase the resistance from V alginolyticus infection.

• 出版日期2008-2-15