Hydrogen-rich saline attenuates hippocampus endoplasmic reticulum stress after cardiac arrest in rats

作者：Gao, Yu; Gui, Qinfang; Jin, Li; Yu, Pan; Wu, Lin; Cao, Liangbin; Wang, Qiang^*; Duan, Manlin^*

来源：Neuroscience Letters, 2017, 640: 29-36.

DOI：10.1016/j.neulet.2017.01.020

• Hydrogen
• Endoplasmic reticulum stress
• Oxidative stress
• CPR
• Apoptosis

Background: Hydrogen-rich saline can selectively scavenge reactive oxygen species (ROS) and protect brain against ischemia reperfusion (I/R) injury. Endoplasmic reticulum stress (ERS) has been implicated in the pathological process of cerebral ischemia. However, very little is known about the role of hydrogen rich saline in mediating pathophysiological reactions to ERS after I/R injury caused by cardiac arrest. @@@ Methods: The rats were randomly divided into three groups, sham group (n =30), ischemia/reperfusion group (n= 40) and hydrogen-rich saline group (n=40). The rats in experimental groups were subjected to 4 min of cardiac arrest and followed by resuscitation. Then they were randomized to receive 5 ml/kg of either hydrogen-rich saline or normal saline. @@@ Results: Hydrogen-rich saline significantly improves survival rate and neurological function. The beneficial effects of hydrogen-rich saline were associated with decreased levels of oxidative products, as well as the increased levels of antioxidant enzymes. Furthermore, the protective effects of hydrogen-rich saline were accompanied by the increased activity of glucose-regulated protein 78 (GRP78), the decreased activity of cysteinyl aspartate specific proteinase-12 (caspase-12) and C/EBP homologous protein (CHOP). @@@ Conclusions: Hydrogen-rich saline attenuates brain I/R injury may through inhibiting hippocampus ERS after cardiac arrest in rats.

• 出版日期2017-2-15
• 单位南京大学