Draft genome sequence of a tigecycline-resistant KPC-2-producing Klebsiella pneumoniae ST15 clinical isolate from China

作者：Xu, Juan; Gao, Meng; Hong, Yan; He, Fang^*

来源：Journal of Global Antimicrobial Resistance, 2017, 11: 156-158.

DOI：10.1016/j.jgar.2017.10.017

• Klebsiella pneumonia
• Tigecycline-resistant
• bla(KPC-2)
• Bioinformatics analysis

Objectives: Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections have emerged worldwide as a serious challenge to public health. Tigecycline is regarded as one of the last-resort antimicrobials for CRKP infections. Here we report the draft genome sequence of a clinical tigecycline-resistant KPC-2-producing K. pneumoniae isolate (TRKP1). @@@ Methods: Whole-genome sequencing of strain TRKP1 was performed using an Illumina Hiseq (TM) X Ten system. The generated reads were assembled using CLC Genomics Workbench. Functional annotation was performed by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) server. Bioinformatics analysis was further performed. @@@ Results: Klebsiella pneumoniae TRKP1 belongs to sequence type ST15, corresponding to clonal complex CC23. The total genome length of the isolate was 5 609 823 bp, harbouring 19 genes relevant to antibiotic resistance. Antimicrobial susceptibility testing revealed that the isolate is resistant to all antibiotics except colistin. @@@ Conclusions: The genome sequence of K. pneumoniae TRKP1 will facilitate the understanding of drug resistance mechanisms and genomic features of clinically isolated tigecycline-resistant CRKP.

• 出版日期2017-12
• 单位浙江省人民医院; 浙江省医学科学院