MAPK3/1 is conducive to luteinizing hormone-mediated C-type natriuretic peptide decrease in bovine granulosa cells

作者：Yang, Lei; Wei, Qiang; Ge, Junbang; Zhao, Xiaoe; Ma, Baohua^*

来源：Journal of Reproduction and Development, 2016, 62(2): 137-142.

DOI：10.1262/jrd.2015-135

• C-type natriuretic peptide (CNP)
• Epidermal growth factor receptor (EGFR)
• Luteinizing hormone (LH)
• Mitogen-activated protein kinase3/1 (MAPK3/1)

C-type natriuretic peptide (CNP) plays a role as an oocyte maturation inhibitor (OMI) in many species, including the bovine. However, the effects of luteinizing hormone (LH) on CNP expression and its potential mechanisms have not reported in the bovine. In the present study, we aimed to study the effects of LH on CNP expression and to illuminate the potential molecular mechanism in this process. Our results showed that LH induced epidermal growth factor receptor (EGFR) phosphorylation, mitogen-activated protein kinase3/1 (MAPK3/1) activation and CNP mRNA decrease in cultured bovine granulosa cells. Further study revealed that LH suppressed CNP expression via the MAPK3/1 signaling pathway, which was activated by the EGFR pathway. In conclusion, our research suggested that MAPK3/1 is involved in LH-mediated decrease of CNP and that this process is related to the EGFR and MAPK3/1 signal pathways.

• 出版日期2016-4
• 单位西北农林科技大学