Assembly of kafirin/carboxymethyl chitosan nanoparticles to enhance the cellular uptake of curcumin

作者：Xiao Jie; Nian Si; Huang Qingrong^*

来源：Food Hydrocolloids, 2015, 51: 166-175.

DOI：10.1016/j.foodhyd.2015.05.012

• Kafirin
• Carboxymethyl-chitosan
• Curcumin
• Nanoparticles
• Self-assembly
• Cellular uptake

Kafirin, the prolamin protein from sorghum, has been utilized to form two types of nanocapsules to enhance the cellular uptake of curcumin: (1) kafirin based colloidal particles containing curcumin (cc-kaf) prepared by solvent induced kafirin self-assembly; and (2) kafirin/carboxymethyl-chitosan nanoparticles (cc-kaf/CMC) prepared by acid-induced aggregation. Both nanocapsules showed spherical morphology with cc-kaf/CMC presenting larger average particle sizes as determined by dynamic light scattering (DLS) and atomic force microscopy (AFM). The encapsulation efficiency and loading efficiency were 55.0 +/- 1.1% and 5.0 +/- 0.1% for cc-kaf and increased to 86.1 +/- 2.1% and 6.1 +/- 0.2% after introducing CM-chitosan. Hydrogen bonding, electrostatic attraction and hydrophobic interaction were found to be the major driving forces for particle formulation and curcumin encapsulation. The involvement of CM-chitosan improved the photo stability, dissolution profile in simulated gastrointestinal tract and cellular uptake of curcumin. A schematic illustration of the formation of cc-kaf and cc-kaf/CMC and their corresponding performance were proposed. The designed methodology suits particularly for the encapsulation of water-insoluble nutraceuticals with the enhanced encapsulation efficiency and nutraceutical uptake.

• 出版日期2015-10