A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection

作者：Yan Junhong; Horak Daniel^*; Lenfeld Jiri; Hammond Maria; Kamali Moghaddam Masood

来源：Journal of Biotechnology, 2013, 167(3): 235-240.

DOI：10.1016/j.jbiotec.2013.06.010

• Bead cellulose
• Magnetic
• Protein detection
• Proximity ligation assay

Magnetic bead cellulose (MBC) was prepared using sol-gel transition of viscose in the presence of maghemite (gamma-Fe2O3) nanoparticles. The MBC particles were then activated with p-toluenesulfonyl chloride to yield tosyl-activated magnetic bead cellulose (MBC-Ts). The microspheres were characterized by light and electron microscopy, elemental analysis and atomic absorption spectroscopy to determine morphology, size, polydispersity and content of iron and tosyl groups. The functionality of the MBC-Ts microspheres was demonstrated using proximity ligation assay (PLA) to detect vascular endothelial growth factor in femtomolar concentration range. The MBC-Ts microspheres performed equally well as commercially available microparticles that are routinely used as solid support in solid phase PLA.

• 出版日期2013-9-10