Gonadorelin at the outset and/or end of an ovulation synchronization progesterone estradiol benzoate-based protocol in Nellore females

作者：Campos Carla Cristian^*; Oliveira Mayara; Ferreira Mohallem Renata de Freitas; dos Santos Ricarda Maria

来源：Semina-Ciencias Agrarias, 2016, 37(1): 173-181.

DOI：10.5433/1679-0359.2016v37n1p173

• Gonadotropin-releasing hormone
• Nellore
• pregnancy
• synchronization
• timed AI

This study aimed to evaluate the effects of gonadorelin (GnRH), used both at the outset of a timed artificial insemination (TAI) protocol to synchronize follicular wave recruitment and at the end to synchronize ovulation, on the conception rate (CR) in Nellore cows. The experiment was conducted on two beef cattle farms in the Vale do Araguaia, MT. The body condition score (BCS) evaluation and ultrasound examination to determine ovarian status [follicle diameter < 10 mm, >= 10 mm or presence of corpus luteum (CL)] were performed at the beginning (Day 0) of the TAI protocol. Multiparous cows (n = 494) were subjected to the following protocol: Day 0 - insertion of intravaginal progesterone device (P-4 DIB (R), MSD) previously used for 8 or 16 days and IM administration of 2.0 mg of estradiol benzoate (EB, Gonadiol (R), MSD); Day 8 - P4 device withdrawal, IM injection of 300 IU of equine chorionic gonadotropin (eCG, Folligon (R), MSD), 1.0 mg of estradiol cypionate (ECP (R), Zoetis) and 0.265 mg of Sodium cloprostenol (PGF(2a), Ciosin (R), MSD); Day 10 - TAI, performed by a single inseminator. Animals were randomly assigned to one of four groups: control (n = 126), GnRH D0 (n = 123), GnRH D10 (n = 123) and GnRH D0 + D10 (n = 122). Animals treated with GnRH received 50 mcg of gonadorelin (GnRH, Cystorelin (R), Merial). Pregnancy was diagnosed by ultrasound 39 +/- 10 days after TAI. Data were analyzed using the GLIMMIX procedure of SAS program (P < 0.05). The overall CR was 42.71%. Effect of treatment was not detected (P = 0.2482) on CR, which was 36.89% +/- 0.19 (47/126) for the control group, 46.08% +/- 0.19 (56/123) for GnRH D0, 48.30% +/- 0.18 (61/123) for GnRH D10 and 38.48% +/- 0.19 (47/122) for GnRH D0 + D10. An effect of farm was detected; the CR was 47.32% +/- 0.13 at Farm A and was 38.24% +/- 0.13 at Farm B (P = 0.0249). No effects of the interaction between farm and treatment were observed (P = 0.7662), nor of those among ovarian condition and treatment (P = 0.1225) on CR. Cows with follicles < 10 mm had lower CR (16.44% +/- 0.08; P = 0.0001) than cows with follicles = 10 mm (46.15% +/- 0.07) and cows with CL (48.35% +/- 0.07). It was concluded that GnRH used to both synchronize the follicular wave recruitment and synchronize ovulation as part of a TAI protocol, did not improve the conception rate in Nellore beef cows.

• 出版日期2016