Autophagy Inhibition Stimulates Apoptosis in Oesophageal Squamous Cell Carcinoma Treated with Fasudil

作者：Xie, Fa-Jun^*; Zheng, Qiu-Qing; Qin, Jing; Zhang, Ling-Ling; Han, Na; Mao, Wei-Min^*

来源：Journal of Cancer, 2018, 9(6): 1050-1056.

DOI：10.7150/jca.23388

• Fasudil
• Oesophageal squamous cell carcinoma
• Apoptosis
• Autophagy

Fasudil has been proven to be a promising chemotherapeutic drug for various malignancies. However, the potential anticancer effects of fasudil in oesophageal squamous cell carcinoma (ESCC) remain to be established. We confirmed the RhoA activity is inhibited by fasudil in ESCC cells. Then measured the effects of fasudil on apoptosis and autophagy in ESCC. Our study showed fasudil could both induce ESCCs apoptosis and autophagy, and when fasudil-induced autophagy was inhibited by knockdown of the essential autophagy genes (Beclin 1 or ATG7), and pharmacologic agent (chloroquine) treatment, both treatments also significantly sensitized ESCC to fasudil-induced apoptosis, reducing cell viability in vitro. Our study showed autophagy inhibitors combined with fasudil could significantly induce ESCC apoptosis, which may provide a novel therapeutic strategy for ESCC.

• 出版日期2018
• 单位浙江省肿瘤医院; 北京大学