A sensitivity-improved enzyme-linked immunosorbent assay for fenvalerate: a new approach for hapten synthesis and application to tea samples

作者：Song, Yang; Lu, Yang; Liu, Bing; Xu, Na; Wang, Shuo^*

来源：Journal of the Science of Food and Agriculture, 2011, 91(12): 2210-2216.

DOI：10.1002/jsfa.4441

• fenvalerate
• polyclonal antibody
• ELISA
• matrix effect
• cross-reactivity

BACKGROUND: Fenvalerate has been widely used for the control of many common pests, but residues of this pesticide have been found in some agricultural crops. China is a large exporter of tea products; thus monitoring of pesticide residues in tea products has become increasingly important. In this study, a method of competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for the rapid detection of fenvalerate in tea sample was developed.
RESULTS: A polyclonal antibody against fenvalerate (FEN) was produced by the hapten with the characteristic moiety of fenvalerate. After acidification, the hapten was synthesized from 2-(4-chloro-phenyl)-3-methyl-butyric acid and aminocaproic acid methyl ester. The CD-ELISA method developed has a high sensitivity of detection: 9 mu g L-1 for IC50 and 0.5 mu g L-1 for IC15. Fenvalerate was treated with 0.5 mmol L-1 NaOH-methanol solution to improve its solubility by isomerization. In the tea sample, the detection limit of fenvalerate was 0.16 mg L-1. A recovery rate of 76.67-91.43% was obtained from spiked tea. The reliability of the CD-ELISA method is better in comparison with the gas chromatographic method (R-2 = 0.9968).
CONCLUSION: In this study, a simple and efficient immunoassay method was developed. It is preferable for the rapid determination of fenvalerate residues in tea samples.

• 出版日期2011-9
• 单位天津科技大学