A 10 000-fold Nuclear Hyperpolarization of a Membrane Protein in the Liquid Phase via Solid-State Mechanism

作者：Daviso Eugenio; Janssen Geertje Jacoba; Alia A; Jeschke Gunnar; Matysik Jorg^*; Tessari Marco

来源：Journal of the American Chemical Society, 2011, 133(42): 16754-16757.

DOI：10.1021/ja206689t

Several techniques rely on electron-nuclear interactions to boost the polarization of nuclear spins in the solid phase. Averaging out of anisotropic interactions as a result of molecular tumbling strongly reduces the applicability of such hyperpolarization approaches in liquids. Here we show for the first time that anisotropic electron-nuclear interactions in solution can survive sufficiently long to generate nuclear spin polarization by the solid-state photo-CIDNP mechanism. A 10 000-fold NMR signal increase in solution was observed for a giant biomolecular complex of a photosynthetic membrane protein with a tumbling correlation time in the submicrosecond regime, corresponding to a molecular weight close to 1 MDa.

• 出版日期2011-10-26