Acute and subacute oral toxicity of polychlorinated diphenyl sulfides in mice: Determining LD50 and assessing the status of hepatic oxidative stress

作者：Zhang Xuesheng; Liu Fu; Chen Binyuan; Li Ying; Wang Zunyao^*

来源：Environmental Toxicology and Chemistry, 2012, 31(7): 1485-1493.

DOI：10.1002/etc.1861

• Polychlorinated diphenyl sulfides
• Median lethal dose
• Toxicity
• Histological analysis
• Oxidative stress

Polychlorinated diphenyl sulfides (PCDPSs), a series of dioxin-like compounds, have been detected in various environmental samples. However, information on the toxicity of these compounds is limited. In the present study, the toxic effects of PCDPSs were assessed after acute and subacute exposure in mice. Relationships between acute toxicity, number, and position of substituted Cl atoms were assessed. In the acute study, 11 types of PCDPSs were administered to female Kunming mice by gavage, and median lethal doses (LD50s) were determined by the Karber method. Results indicated that the LD50s of lower substituted PCDPSs were smaller than higher substituted PCDPSs. Substituted positions also influenced the LD50 of PCDPSs. Terminal necropsy showed increased relative liver weights and decreased relative kidney weights. Histological examination of livers demonstrated swollen cells, inflammation, vacuolization, and necrosis. In the 28-d subacute exposure tests, 11 types of PCDPSs were dissolved in corn oil and administered to mice at doses of 1, 10, and 100?mg/kg. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels in mouse liver were determined after the exposure period. Results suggested that lower substituted PCDPSs decreased SOD activity in the high-dose groups compared with controls, and MDA level in the 100-mg/kg dose group was significantly increased. In addition, acute toxicity of PCDPSs partly corresponded to the hepatic oxidative damage observed. Environ. Toxicol. Chem. 2012; 31: 14851493.

• 出版日期2012-7
• 单位南京大学; 常州大学