Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold

作者：Klangjorhor Jeerawan; Nimkingratana Puwapong; Settakom Jongkolnee; Pruksakom Dumnoensun; Leerapun Taninnit; Ar****chayanon Olarn; Rojanasthien Sattaya; Kongtawelert Prachya; Pothacharoen Peraphan^*

来源：Journal of Orthopaedic Surgery and Research, 2012, 7(1): 40.

DOI：10.1186/1749-799X-7-40

• Human articular chondrocyte 3D culture
• Hyaluronan
• Collagen
• Gelatin
• Spongostan
• Scaffold

Background: Autologous chondrocyte transplantation is a promising technique for treatment of cartilage defects. Three dimensional chondrocyte cultures on a scaffold are widely used to retain the chondrogenic phenotype. Using a biodegradable gelatin scaffold is one option for the cell delivery system, but molecular and histological studies of the method have not yet been done. %26lt;br%26gt;Methods: We evaluated the chondrogenic property of the primary human chondrocyte on a gelatin scaffold as compared to a collagen scaffold over a period of 21 days. We examined the production of glycosaminoglycan by quantitative and histological analysis. Gene expression of cartilage-associated molecules was assessed by quantitative RT-PCR. %26lt;br%26gt;Results: The gelatin scaffold showed the ability to promote chondrocyte expansion, chondrogenic phenotype retention at molecular and mRNA levels. %26lt;br%26gt;Conclusions: This scaffold is thus suitable for use as an in vitro model for chondrocyte 3D culture.

• 出版日期2012-12-19