A novel digital method to assess air space loss associated with acute lung injury in experimental acute pancreatitis

作者：Mukherjee, R.^*; Wen, L.; Zhang, X.; Bhattacharya, P.; Huang, W.; Sutton, R.

来源：Pancreatology, 2018, 18(5): 513-515.

DOI：10.1016/j.pan.2018.04.017

• Acute lung injury
• Acute pancreatitis

Background/Objectives: Respiratory dysfunction and/or failure from acute lung injury (ALI) are common in acute pancreatitis (AP), but assessment of ALI in experimental AP has lacked standardisation.
Methods: A range of experimental AP models induced in C57BL/6 mice with corresponding controls (n = 6/group). Full double lung or right lung specimens were taken for histopathological assessment and slides analysed by a pre-set pipeline using Aperio Scanner (Leica), ImageJ software and CellProfiler software. Findings were compared to other routinely assessed parameters.
Results: Overall histopathological changes were similar between both lungs. Mean lung field occupancy was significantly different between moderate and severe CER-AP (21.9% v 27.5%, p < 0.05) and corresponded with lung MPO and local injury severity parameters and was mirrored for all models tested.
Conclusion: We have developed a novel, simple method for assessment of ALI to improve measurement of systemic organ injury in experimental AP and contribute to preclinical drug development.

• 出版日期2018-7
• 单位四川大学