alpha B-Crystallin Polydispersity Is a Consequence of Unbiased Quaternary Dynamics

作者：Baldwin Andrew J^*; Lioe Hadi; Robinson Carol V; Kay Lewis E; Benesch Justin L P

来源：Journal of Molecular Biology, 2011, 413(2): 297-309.

DOI：10.1016/j.jmb.2011.07.016

• Polydispersity
• mass spectrometry
• small heat shock proteins (sHSPs)
• molecular chaperones
• thermodynamics and kinetics

The inherent heterogeneity of many protein assemblies complicates characterization of their structure and dynamics, as most biophysical techniques require homogeneous preparations of isolated components. For this reason, quantitative studies of the molecular chaperone alpha B-crystallin, which populates a range of interconverting oligomeric states, have been difficult, and the physicochemical basis for its polydispersity has remained unknown. Here, we perform mass spectrometry experiments to study alpha B-crystallin and extract detailed information as to its oligomeric distribution and exchange of subunits under a range of conditions. This allows a determination of the thermodynamic and kinetic parameters that govern the polydisperse ensemble and enables the construction of a simple energy profile for oligomerization. We find that the quaternary structure and dynamics of the protein can be explained using a simple model with just two oligomer-independent interactions (i.e., interactions that are energetically identical in all oligomers from 10mers to 40mers) between constituent monomers. As such, the distribution of oligomers is governed purely by the dynamics of individual monomers. This provides a new means for understanding the polydispersity of aB-crystallin and a framework for interrogating other heterogeneous protein assemblies.

• 出版日期2011-10-21