A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles

作者：Chen, Mingjian; Xiang, Xinying; Wu, Kefeng; He, Hailun; Chen, Hanchun; Ma, Changbei^*

来源：Sensors (Switzerland), 2017, 17(11): 2684.

DOI：10.3390/s17112684

• label-free
• human serum albumin
• copper nanoparticles

In this work, we developed a facile fluorescence method for quantitative detection of human serum albumin (HSA) based on the inhibition of poly(thymine) (poly T)-templated copper nanoparticles (CuNPs) in the presence of HSA. Under normal circumstances, poly T-templated CuNPs can display strong fluorescence with excitation/emission peaks at 340/610 nm. However, in the presence of HSA, it will absorb cupric ion, which will prevent the formation of CuNPs. As a result, the fluorescence intensity will become obviously lower in the presence of HSA. The analyte HSA concentration had a proportional linear relationship with the fluorescence intensity of CuNPs. The detection limit for HSA was 8.2 x 10(-8) molL(-1). Furthermore, it was also successfully employed to determine HSA in biological samples. Thus, this method has potential applications in point-of-care medical diagnosis and biomedical research.

• 出版日期2017-11
• 单位中南大学