A quantitative assay for Amaranthus palmeri identification

作者：Murphy Brent P; Plewa Diane E; Phillippi Elizabeth; Bissonnette Suzanne M; Tranel Patrick J^*

来源：Pest Management Science, 2017, 73(11): 2221-2224.

DOI：10.1002/ps.4632

• Amaranthus palmeri
• species identification
• qPCR
• Conservation Reserve Program

BACKGROUNDAmaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement.
RESULTSMarkers were developed for A. palmeri-specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single-plant samples, simulated mixed-plant samples and seed mixtures.
CONCLUSIONA qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds.

• 出版日期2017-11