Acetylene-sourced CVD-synthesised catalytically active graphene for electrochemical biosensing

作者：Osikoya Adeniyi Olugbenga; Parlak Onur; Murugan N Arul; Dikio Ezekiel Dixon; Moloto Harry; Uzun Lokman; Turner Anthony P F; Tiwari Ashutosh^*

来源：Biosensors and Bioelectronics, 2017, 89: 496-504.

DOI：10.1016/j.bios.2016.03.063

• CVD-grown graphene
• bioelectronics
• theoretical calculation
• surfactant modification
• 2d-materials

In this study, we have demonstrated the use of chemical vapour deposition (CVD) grown-graphene to develop a highly-ordered graphene-enzyme electrode for electrochemical biosensing. The graphene sheets were deposited on 1.00 mm thick copper sheet at 850 degrees C using acetylene (C2H2) as carbon source in an argon (Ar) and nitrogen (N-2) atmosphere. An anionic surfactant was used to increase wettability and hydrophilicity of graphene; thereby facilitating the assembly of biomolecules on the electrode surface. Meanwhile, the theoretical calculations confirmed the successful modification of hydrophobic nature of graphene through the anionic surface assembly, which allowed high-ordered immobilisation of glucose oxidase (GOx) on the graphene. The electrochemical sensing activities of the graphene-electrode was explored as a model for bioelectrocatalysis. The bioelectrode exhibited a linear response to glucose concentration ranging from 0.2 to 9.8 mM, with sensitivity of 0.087 A/M/cm(2) and a detection limit of 0.12 mu M (S/N=3). This work sets the stage for the use of acetylene-sourced CVD-grown graphene as a fundamental building block in the fabrication of electrochemical biosensors and other bioelectronic devices.

• 出版日期2017-3-15