A novel DNA restriction technology based on laser pulse energy conversion on sequence-specific bound metal nanoparticles

作者：Csaki A; Maubach G; Garwe F; Steinbruck A; Konig K; Fritzsche W^*

来源：Conference on Imaging, Manipulation, and Analysis of Biomolecules and Cells - Fundamentals and Applications III, 2005-01-24 to 2005-01-27.

DOI：10.1117/12.590450

• DNA
• restriction
• laser
• metal nanoparticles
• gold
• metaphase chromosomes

DNA restriction is a basic method in today's molecular biology. Besides application for DNA manipulation, this method is used in DNA analytics for 'restriction analysis'. Thereby DNA is digested by sequence specific restriction enzymes, and the length distribution of the resulting fragments is detected by gel electrophoresis. Differences in the sequence lead to different restriction patterns. A disadvantage of this standard method is the limitation to a small set of fixed sequences, so that the assay can not be adapted to any sequence of interest (e.g. SNP).
We designed a scheme for DNA restriction in order to provide access to any desired sequence, based on laser light conversion on sequence-specific positioned metal nanoparticles. Especially gold nanoparticles are known for their interesting optical proper-ties caused by plasmon resonance. The resulting absorption can be used to convert laser light pulses into heat, resulting in nanoparticle destruction. We work on the combination of this principle with DNA-modification of nanoparticles and the sequence-specific binding (hybridization) of these DNA-nanoparticle complexes along DNA molecules. Different mechanisms of light-conversion were studied, and the destructive effect of laser light on the nanoparticles and DNA is demonstrated.

• 出版日期2005