Development of a Xanthene-Based Red-Emissive Fluorescent Probe for Visualizing H2O2 in Living Cells, Tissues and Animals

作者：Zhang, Nan; Dong, Baoli; Kong, Xiuqi; Wang, Chao; Song, Wenhui; Lin, Weiying^*

来源：Journal of Fluorescence, 2018, 28(2): 681-687.

DOI：10.1007/s10895-018-2231-6

• Fluorescent probe
• Hydrogen peroxide
• DFT calculations
• In vivo imaging

Hydrogen peroxide (H2O2) plays important roles in the regulation of many biological processes, and the abnormal level of H2O2 has close relation with the initiation and progression of many diseases. Herein, we describe a novel red-emissive fluorescence probe (RhoB) for the visualization of H2O2 in living cells, tissues and animals. RhoB was constructed on the basis of a xanthene-based red-emissive dye, and displayed nearly no fluorescence. After the treatment with H2O2, RhoB can exhibit red fluorescence with the emission wavelength at 638 nm. RhoB exhibited highly sensitive and selective response to H2O2. Density functional theory (DFT) calculations were conducted to shed light on the optical properties of RhoB, and natural bond orbital (NBO) calculations demonstrate that the boron atom shows the highest positive electricity and further support the response mechanism. RhoB was successfully applied for imaging of exogenous and endogenous H2O2 in living cells, and also can be utilized for visualizing H2O2 in living tissues and animals.

• 出版日期2018-3
• 单位济南大学