A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum

作者：Yoshida Ayako^*; Nagayasu Eiji; Horii Yoichiro; Maruyama Haruhiko

来源：Parasitology Research, 2012, 110(4): 1583-1586.

DOI：10.1007/s00436-011-2677-9

C-type lectins (CTLs) are a group of proteins which bind to carbohydrate epitopes in the presence of Ca2+, which have been described in a wide range of species. In this study, a cDNA sequence coding a putative CTL has been identified from the cDNA library constructed from the pig round worm Ascaris suum lung L3 (LL3) larvae, which was designated as A. suum C-type lectin-1 (As-CTL-1). The 510 nucleotide open reading frame of As-CTL-1 cDNA encoded the predicted 169 amino acid protein including a putative signal peptide of 23 residues and C-type lectin/C-type lectin-like domain (CLECT) at residue 26 to 167. As-CTL-1 was most similar to Toxocara canis C-type lectin-1 and 4 (Tc-CTL-1 and 4), and highly homologous to namatode CTLs and mammalian CTLs as well, such as human C-type lectin domain family 4 member G (CLECG4). In addition, As-CTL-1 was strongly expressed in tissue migrating LL3 and the L4 larvae, which were developmental larvae stages within the mammalian host. These results suggest that A. suum larvae might utilize As-CTL-1 to avoid pathogen recognition mechanisms in mammalian hosts due to it is similarity to host immune cell receptors.

• 出版日期2012-4