alpha-Lipoic Acid Reduces Fatty Acid Esterification and Lipogenesis in Adipocytes from Overweight/Obese Subjects

作者：Fernandez Galilea Marta; Perez Matute Patricia; Prieto Hontoria Pedro L; Sainz Neira; Lopez Yoldi Miguel; Houssier Marianne; Martinez J Alfredo; Langin Dominique; Moreno Aliaga Maria J^*

来源：Obesity, 2014, 22(10): 2210-2215.

DOI：10.1002/oby.20846

Objective-Lipoic acid (-LA) is a natural occurring antioxidant with beneficial effects on obesity. The aim of this study was to investigate the putative effects of -LA on triglyceride accumulation and lipogenesis in subcutaneous adipocytes from overweight/obese subjects and to determine the potential mechanisms involved. %26lt;br%26gt;MethodsFully differentiated human subcutaneous adipocytes were treated with -LA (100 and 250 mu M) during 24 h for studying triglyceride content, de novo lipogenesis, and levels of key lipogenic enzymes. The involvement of AMP-activated protein kinase (AMPK) activation was also evaluated. %26lt;br%26gt;Results-LA down-regulated triglyceride content by inhibiting fatty acid esterification and de novo lipogenesis. These effects were mediated by reduction in fatty acid synthase (FAS), stearoyl-coenzyme A desaturase 1, and diacylglycerol O-acyltransferase 1 protein levels. Interestingly, -LA increased AMPK and acetyl CoA carboxylase phosphorylation, while the presence of the AMPK inhibitor Compound C reversed the inhibition observed on FAS protein levels. %26lt;br%26gt;Conclusions-LA down-regulates key lipogenic enzymes, inhibiting lipogenesis and reducing triglyceride accumulation through the activation of AMPK signaling pathway in human subcutaneous adipocytes from overweight/obese subjects.

• 出版日期2014-10