An ultra-small three dimensional computational microscope

作者：Kim Ganghun; Menon Rajesh^*

来源：Applied Physics Letters, 2014, 105(6): 061114.

DOI：10.1063/1.4892881

We present a computational approach that allows one to create microscopic images in 3D through a miniature, rigid cannula. Light rays propagate from one end of the cannula to the other resulting in a complex intensity distribution. This distribution is unique to the position of the source of the rays. By applying appropriate calibration and utilizing a nonlinear optimization algorithm, we computationally reconstructed images of objects with a minimum feature size of similar to 5 mu m. Preliminary experiments indicate that the sensitivity of the imaging technique can be as small as 100 nm in the transverse plane and similar to 1 mu m in the longitudinal direction. Since the cannula is only 14.7 mm long and 200 mu m in diameter, this allows for highly miniaturized microscopes that utilize no optics and no scanning. Furthermore, since the images are obtained with just a single frame (no scanning), fast image acquisition is also feasible.

• 出版日期2014-8-11