Augmentation of hypoxia-inducible factor-1-alpha in reinfused blood cells enhances diabetic ischemic wound closure in mice

作者：Wang, Huan; Feng, Yufeng; Jin, Xiaoju; Xia, Rong; Cheng, Yong; Liu, Xiaoqian; Zhu, Nana; Zhou, Xun; Yin, Lei; Guo, Jianrong^*

来源：Oncotarget, 2017, 8(69): 114251-114258.

DOI：10.18632/oncotarget.23214

• diabetic wound closure
• blood re-infusion
• hypoxia-inducible factor-1 alpha (HIF-1 alpha)
• vascular endothelial growth factor ( VEGF)

Diabetes-associated dysfunction in angiogenesis predominantly contributes to impairment of wound closure, but a role of hypoxia-inducible factor 1 alpha (HIF-1a) in the process remain poorly understood. Here, we examined whether expression of HIF-1a in re-infused blood cells may improve the diabetic wound closure in mice. We found that that expression of HIF-1a in re-infused isogeneic blood cells significantly improved diabetic wound healing in mice, seemingly through augmentation of wound-associated angiogenesis. Mechanistically, expression of HIF-1a in re-infused blood cells significantly increased macrophage infiltration at the wound site, and macrophages produced vascular endothelial growth factor A (VEGF-A) to promote wound-associated angiogenesis. Together, our data suggest that augmentation of HIF-1a in reinfused blood cells may enhance diabetic ischemic wound closure.

• 出版日期2017-12-26
• 单位上海市浦东新区公利医院; 中国人民解放军第二军医大学; 厦门大学; 皖南医学院; 复旦大学