Mastoid Obliteration Using Three-Dimensional Composite Scaffolds Consisting of Polycaprolactone/-Tricalcium Phosphate/Collagen Nanofibers: An In Vitro and In Vivo Study

作者：Jang Chul Ho^*; Cho Yong Beom; Yeo Myung Gu; Kim Geun Hyung

来源：Macromolecular Bioscience, 2013, 13(5): 660-668.

DOI：10.1002/mabi.201200393

• beta-tricalcium phosphate
• collagen
• mastoid obliteration
• nanofiber polycaprolactone

Two different composite scaffolds, solid-freeform-fabricated PCL/-TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/-TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular activities of osteoblast-like-cells (MG63), SEM images and MTT assays are conducted. Experimental mastoid obliteration is performed using guinea pigs that are divided group A (PCL/-TCP/collagen-nanofiber scaffold) and group B (PCL/-TCP scaffold). The results reveal that PCL/-TCP/collagen scaffold provide much broader cell attachment sites than PCL/-TCP scaffold. The mu-CT and fluorescent microscopy results reveal that the acceleration of early new bone formation within the pores and scaffold itself at week 4 post-operation is more effective in group A. In addition, based on the results of the histological and mu-CT at 12 weeks post-surgery, the effective regeneration of bone in the PCL/-TCP/collagen scaffold is appeared.

• 出版日期2013-5