科研之友
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摘要
Traditional methods for regulating oxygen concentration ([O-2]) in in vitro experiments over the range found in normal and tumor tissues require the use of expensive equipment to generate controlled gas atmospheres or the purchase of a range of gas cylinders with certified O-2 percentages. Here we describe a simple and inexpensive enzymatic method for generating low, precise steady-state [O-2] levels that are stable for several hours. This method is particularly applicable to the in vitro study of some classes of hypoxia-targeted antitumor prodrugs and bioreductively activated agents.
- 出版日期2015-12-15
