Apoptotic bone marrow CD34+cells in cirrhotic patients

作者：Dang Shuang Suo^*; Wang Wen Jun; Gao Ning; Wang Shun Da; Li Mei; Liu La Yang; Sun Ming Zhun; Dong Tao

来源：World Journal of Gastroenterology, 2011, 17(15): 2044-2048.

DOI：10.3748/wjg.v17.i15.2044

• Cirrhosis
• CD34
• Hematopoietic stem cells/Hematopoietic progenitor cells
• Apoptosis

AIM: To access the frequency and level of apoptotic CD34+ cells isolated from the marrow fluid of patients with post-hepatitis cirrhosis.
METHODS: The frequency of bone marrow CD34+ cells and apoptotic bone marrow CD34+ cells in 31 inpatients with post-hepatitis cirrhosis (cirrhosis group), and 15 out-patients without liver or blood disorders (control group) was calculated by flow cytometry. Parameters were collected to evaluate liver functions of patients in cirrhosis group.
RESULTS: The percentage of normal bone marrow CD34+ cells was 6.30% +/- 2.48% and 1.87% +/- 0.53% (t = 3.906, P < 0.01) while that of apoptotic marrow CD34+ cells was 15.00% +/- 15.81% and 5.73% +/- 1.57% (t = 2.367, P < 0.05) in cirrhosis and control groups, respectively. The percentage of apoptotic marrow CD34+ cells was 6.25% +/- 3.30% and 20.92 +/- 18.5% (t = 2.409, P < 0.05) in Child-Pugh A and Child-Pugh B + C cirrhotic patients, respectively. The percentage of late apoptotic marrow CD34+ cells was positively correlated with the total bilirubin and aspartate aminotransferase serum levels in patients with cirrhosis.
CONCLUSION: The status of CD34+ marrow cells in cirrhotic patients may suggest that the ability of hematopoietic progenitor cells to transform into mature blood cells is impaired.

• 出版日期2011-4-21
• 单位西安交通大学