A Description of Cochliopodium megatetrastylus n. sp Isolated from a Freshwater Habitat

作者：Anderson O Roger^*; Tekle Yonas I

来源：Acta Protozoologica, 2013, 52(2): 55-64.

DOI：10.4467/16890027AP.13.006.1085

• Amoebozoa
• comparative morphology
• fine structure
• molecular genetics
• taxonomy

Cochliopodium megatetrastylus n. sp. is described based on light microscopy, fine structure and molecular genetic evidence. Amoebae are broadly oval to somewhat triangular during locomotion with average length of 37 mu m and breadth of 50 mu m, and surrounded by a hyaloplasm margin, somewhat narrow when at rest but more expanded during locomotion (similar to 5-10 mu m at the anterior). Sparsely occurring subpseudopodia, barely emergent from the hyaloplasm, are blunt and finger-like, occasionally becoming adhesive laterally or at the posterior. Cysts develop after 2-3 weeks in culture and are round with a distinct margin, decreasing in size from 20 to 5 mu m during maturation. The granuloplasm contains refractile crystals. A vesicular nucleus (similar to 6 mu m), containing a nucleolus (2-3 mu m), is variable in shape from somewhat lenticular in section to irregularly rounded with undulating or lobed margins. Surface scales (similar to 0.3 mu m in height) have an apical deeply concave funnel-like collar (similar to 0.15 mu m deep), without a spine, composed of radial fine rays and concentric filaments forming a fine mesh, supported on four non-cross-linked styles (similar to 0.2 mu m apart) attached to a round to broadly angular base plate (0.6-1 mu m) with a fine grid texture. Cysts are rounded and enclosed by an organic wall bearing remnants of the scales on its outer surface. Both concatenated analysis of SSU-rDNA and COI genes and comparative morphologies support the designation of Cochliopodium megatetrastylus n. sp. as a new species.

• 出版日期2013