Activation of TGF beta 1 signaling enhances early dopaminergic differentiation in unrestricted somatic stem cells

作者：Khanghahi Akram Mokhtarzadeh; Zeynali Bahman^*; Akhlaghpoor Azimeh; Tafreshi Azita Parvaneh; Krieglstein Kerstin

来源：Neuroscience Letters, 2014, 583: 60-64.

DOI：10.1016/j.neulet.2014.08.055

• TGF beta signaling
• USSC
• Dopaminergic differentiation

So far there is increasing evidence for the involvement of transforming growth factor beta TGF beta (transforming growth factor) in differentiation and maintenance of midbrain dopaminergic neurons. Considering that USSCs (unrestricted somatic stem cells) have the potentials to differentiate into neuron like cells and even dopaminergic neurons and that no evidence available on the role of TGF beta signaling in dopaminergic differentiation of these cells, we investigated the presence of TGF beta signaling components in USSCs and their involvement on USSCs differentiation into early dopaminergic neurons. Our results showed that components of TGF beta signaling were present and functional in undifferentiated USSCs, after which the neurally induced USSCs treated with TGF beta 1 for 3 days resulted in increased expression of beta-tubulin III (a general neuronal marker) and Nurr-1 (an early dopaminergic marker) at both mRNA and protein levels. Consistently, TGF beta inhibition in culture medium by using SB431542 in the presence or absence of TGF beta 1, significantly decreased the expression of both neural markers. We therefore suggest that activation of TGF beta signaling-pathway in neurally induced USSCs enhances neural differentiation with an early dopaminergic phenotype which points at the positive role of the TGF beta signaling pathway toward dopaminergic fate.

• 出版日期2014-11-7