Antimicrobial Effect of the Methanolic Extract Psacalium decompositum on Periodontopathogenic Bacteria

作者：Garcia Palencia P; de la Garza Ramos M A; Galindo Rodriguez S A; Oranday Cardenas A; Leos Rivas C; Verde Star M J; Rivas Morales C^*

来源：International Journal of Pharmacology, 2016, 12(7): 737-742.

DOI：10.3923/ijp.2016.737.742

• Psacallum decompositum
• P.gingivalis
• P.intermedia
• S.mutans
• antimicrobial activity

Background and Objective
Periodontal disease and dental caries are frequent oral illnesses. Both have an important impact on quality of life are of polymicrobial origin and progress slowly. Plants are a valuable resource in health systems in developing countries and a large part of traditional treatments involve the use of plant extracts or their active substances. The objective of this study is to determine the antimicrobial activity of the methanolic extract of Psacalium decompositum using the plate diffusion method in vitro and to determine the minimum inhibitory concentration of Porphyromonas gingivalls, Prevotella intermedia and Streptococcus mutans.
Materials and Methods
The plant was collected and identified, Psacalium decompositum and a methanol extract was prepared by mashing. Phytochemical screening was also performed.The methanol extract was evaluted by the plate diffusion method using different microdilutions in broth against the ATCC strains Porphyromonas gingivalls, Prevotella intermedia and Streptococcus mutans. About 0.2% chlorhexidine was used as a positive control and 5% ethanol as a negative control. The nonparametric statistic, the Kruskal-Wallis test was used to compare the three treatments and the Mann-Whitney testfor each pairof treatments to identify significant differences between them.
Results
The minimum inhibitory concentration of the methanolic extract of P. decompositum was 500 mu g mL(-1) for P. gingivalis and P. intertnedia and 700 mu g mL(-1) for 5 mutans (p < 0.002). The minimum inhibitory concentration for chlorhexidine (positive control) was 900 mu g mL(-1) for the three bacteria studied.
Conclusion
The methanolic extract was active against cariogenic and periodontopathogenic bacteria and represents a natural alternative for the control of these microorganisms that produce oral diseases. The methanolic extract of P.decompositum has antimicrobial activity making it a natural alternative for the treatment of caries and periodontal disease.

• 出版日期2016