Analysis of Reactive Oxygen Species, Ca2+, and Hsp70 in the Gill and Mantle of Clams Ruditapes philippinarum Exposed in Cadmium

作者：Liu Dongwu; Chen Zhiwei^*; Liu Zhenxing

来源：Microscopy Research and Technique, 2013, 76(12): 1297-1303.

DOI：10.1002/jemt.22300

• cadmium
• reactive oxygen species
• calcium ion
• Ruditapes philippinarum
• confocal microscopy

In this study, the probes 2,7-dichlorofluorescein diacetate (H2DCF-DA) and Fluo-3 AM were used to investigate the instantaneous change of reactive oxygen species (ROS) and Ca2+ in the gill and mantle of clams Ruditapes philippinarum exposed in 0.05 mg L-1 Cd2+ with the laser-scanning confocal microscopy. The results indicated that Ca2+ level was declined in the gill and slightly increased in the mantle. The level of ROS was declined in the gill, while the oscillation of ROS level was observed in the mantle. These data revealed that Ca2+ could stimulate mitochondrial activity and enhance the respiratory chain in the gill and mantle. In addition, the expression of Hsp70 was increased in the gill and mantle of clams exposed in 0.05 mg L-1 Cd2+. The change of Ca2+ and ROS level affected the expression of Hsp70 in the gill and mantle. An appropriate method was established to analyze the effects of Cd2+ on ROS, Ca2+, and Hsp70 in the gill and mantle of clams with confocal microscopy. Both confocal microscopy and chemical fluorescent are valuable tools for measurement of time-dependent intracellular ROS and Ca2+ signals. Microsc. Res. Tech. 76:1297-1303, 2013.

• 出版日期2013-12
• 单位山东理工大学