Preparation of morusin from Ramulus mori and its effects on mice with transplanted H-22 hepatocarcinoma

作者：Wan Liang Ze; Ma Bin; Zhang Yu Qing^*

来源：Biofactors, 2014, 40(6): 636-645.

DOI：10.1002/biof.1191

• morusin
• alcohol extract
• HPLC-DAD
• mulberry
• NF-B

In this study, we used branches Ramulus mori from cultivated mulberry Husang-32 (Morus multicaulis Perry) as the experimental material and anhydrous ethanol as the extraction solution to obtain the crude extract from the branch bark. The ethanolic extract was successively purified through a macroporous resin, Sephadex LH-20, and semipreparative reversed-phase high-performance liquid chromatography (RP-HPLC). The high-purity monomer was identified as morusin by HPLC with diode array detection (HPLC-DAD) and its UV spectrum. The contents of morusin exhibited almost no difference between the root and branch bark in Husang-32, and morusin was not detected in the leaves. Morusin is able to inhibit the tumor growth of transplanted H-22 hepatocarcinoma in mice and has no side effects. The fluorescence quantitative real-time PCR (qRT-PCR) results indicate that morusin has a marked inhibitory effect on liver cancer cells through a mechanism that may be related increases in the expression of p53, Survivin, CyclinB1, and Caspase-3 and a decrease in NF- B gene expression. The influence of this compound is more apparent in the Caspase-3 and the NF- B genes.

• 出版日期2014-12
• 单位苏州大学