Molecular Characterization and Antiviral Analyses of Porcine Type III Interferons

作者：Sang Yongming; Rowland Raymond R R; Blecha Frank^*

来源：Journal of Interferon and Cytokine Research, 2010, 30(11): 801-807.

DOI：10.1089/jir.2010.0016

Type III interferons (IFNs) are a family of recently identified antiviral cytokines. One to 3 paralogs have been identified in several species; however, little information is available about type III IFNs in pigs. We have identified 2 porcine type III IFNs, Sus scrofa IFN-lambda 1 (SsIFN-lambda 1) and SsIFN-lambda 3, and determined their tissue expression profile and antiviral activities. Open reading frames of SsIFN-lambda 1 and SsIFN-lambda 3 are 576 and 588 bp, encoding 191 and 195 amino acid preproteins, respectively. In healthy pigs, SsIFN-lambda 3 was primarily expressed in mesenteric lymph nodes and intestine, whereas expression of SsIFN-lambda 1 was found in all tested tissues and was high in mesenteric lymph nodes, intestine, and liver. Porcine cells treated with the viral mimic, dsRNA, robustly increased SsIFN-lambda 3 expression, with epithelial cells generally displaying the greatest response. Conversely, dsRNA-induced mRNA expressions of SsIFN-lambda 1, SsIFN-alpha 1, and SsIFN-beta were relatively weaker and delayed compared with SsIFN-lambda 3. SsIFN-lambda 1 and SsIFN-lambda 3 peptides exerted similar but lower antiviral potency than SsIFN-alpha 1 and SsIFN-beta against a porcine arterivirus and an adenovirus. These findings indicate that pigs have 2 type III IFN paralogs, which have antiviral activity and may serve as targets for modulation of the porcine host-pathogen interaction.

• 出版日期2010-11