ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems

作者：Sorgato Veronica^*; Berger Michel; Emain Charlotte; Vever Bizet Christine; Dinten Jean Marc; Bourg Heckly Genevieve; Planat Chretien Anne

来源：Journal of Biomedical Optics, 2016, 21(6): 065003.

DOI：10.1117/1.JBO.21.6.065003

• calibration
• spectroscopy
• reflectance
• optical properties
• quantification

We have developed an adaptive calibration algorithm and protocol (ACA-Pro) that corrects from the instrumental response of various spatially resolved diffuse reflectance spectroscopy (DRSsr) systems to enable the quantification of absorption and scattering properties based on a Monte Carlo-based look-up-table approach. The protocol involves the use of a calibration reference base built with measurements of a range of different diffusive intralipid phantoms. Moreover, an advanced strategy was established to take into account the experimental variations with an additional measurement of a common solid material, allowing the use of a single calibration reference base for all experiments. The ACA-Pro is validated in contact and noncontact probe-based DRSsr systems. Furthermore, the first results of a setup replacing the probe with a CCD detector are shown to confirm the robustness of the approach.

• 出版日期2016-6
• 单位中国地震局