A RAMP marker linked to the tobacco black shank resistant gene

作者：Liu, X. Z.; Yang, Y. M.; He, C. S.; Li, H. L.; Zhang, H. Y.^*

来源：African Journal of Biotechnology, 2009, 8(10): 2060-2063.

• Tobacco
• black shank resistant gene
• RAMP
• molecular marker

Bulk segregant analysis (BSA) and randomly amplified microsatellite polymorphism (RAMP) were employed to analyze F-2 individuals of the Yunyan 317xHubei 517 to screen and characterize molecular markers linked to black shank resistant gene. A total of 800 arbitrary decamer oligonucleotide primer-pairs were used for RAMP analysis. Primer pair GT (CA) (4)/S89, producing one RAMP marker GT (CA) (4)/S89(550), was tightly linked to the black shank resistant gene. Results of Southern blot suggest that the fragment GT (CA) (4)/S89(550) was existed in Yunyan 317 and resistant plants, and absent in Hubei 517. Linkage analysis was carried out using marker GT (CA) (4)/S89(550) on 752 black shank high-resistant individuals of F-2 progenies from crossing between Yunyan 317 and Hubei 517. Our results indicated that the genetic distances between GT (CA) (4)/S89(550) and black shank resistant gene was 1.4cM.

• 出版日期2009-5-18
• 单位西南林业大学; 中国热带农业科学院