Long-term culture of human odontoma-derived cells with a Rho kinase inhibitor

作者：Uzawa Katsuhiro^*; Kasamatsu Atsushi; Saito Tomoaki; Takahara Toshikazu; Minakawa Yasuyuki; Koike Kazuyuki; Yamatoji Masanobu; Nakashima Dai; Higo Morihiro; Sakamoto Yosuke; Shiiba Masashi; Tanzawa Hideki

来源：Experimental Cell Research, 2016, 347(1): 232-240.

DOI：10.1016/j.yexcr.2016.08.005

• Odontoma
• Long-term culture
• Rho kinase inhibitor
• Telomere
• Mineralization
• Dentin sialophosphoprotein

Because of cellular senescence/apoptosis, no effective culture systems are available to maintain replication of cells from odontogenic tumors especially for odontoma, and, thus, the ability to isolate human odontoma-derived cells (hODCs) for functional studies is needed. The current study was undertaken to develop an approach to isolate hODCs and fully characterize the cells in vitro. The hODCs were cultured successfully with a Rho-associated protein kinase inhibitor (Y-27632) for an extended period with stabilized lengths of the telomeres to sustain a similar phenotype/property as the primary tumoral cells. While the hODCs showed stable long-term expansion with expression of major dental epithelial markers including dentin sialophosphoprotein (DSPP) even in the three-dimensional micro environment, they lack the specific markers for the characteristics of stem cells. Moreover, cells from dental pulp showed significant up-regulation of DSPP when co-cultured with the hODCs, while control fibroblasts with the hODCs did not. Taken together, we propose that the hODCs can be isolated and expanded over the long term with Y-27632 to investigate not only the development of the hODCs but also other types of benign human tumors.

• 出版日期2016-9-10